AOD-9604 is a synthetic peptide derived from the C-terminal fragment of human growth hormone (HGH), specifically amino acids 176–191. Unlike full-length HGH, AOD-9604 was designed to retain fat-burning properties without stimulating insulin-like growth factor 1 (IGF-1) production, which is associated with growth and metabolic side effects.

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Mechanism of Action:

AOD-9604 operates by enhancing lipolysis—the breakdown of fat—while inhibiting lipogenesis, the process of creating new fat. It achieves this by stimulating beta-3 adrenergic receptors in adipocytes (fat cells), which are pivotal in fat metabolism. Notably, studies have shown that AOD-9604 can increase energy expenditure and fat oxidation, even in the absence of these receptors, suggesting alternative pathways for fat burning.

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Clinical Research and Applications:

AOD-9604 has been investigated primarily for its potential in weight management and metabolic health. In animal studies, it demonstrated the ability to reduce body fat without adversely affecting insulin sensitivity. However, human clinical trials have yielded mixed results. A 12-week randomized trial indicated modest weight loss benefits, while a subsequent 24-week trial showed insufficient efficacy, leading to the termination of its development in 2007.

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Safety Profile:

One of the advantages of AOD-9604 is its safety profile. Unlike full HGH, it does not stimulate IGF-1 production, thereby avoiding potential side effects such as organ growth and insulin resistance. Clinical studies have reported that AOD-9604 is well-tolerated, with minimal adverse effects.

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Administration and Usage:

AOD-9604 is typically administered through subcutaneous injections or oral lozenges. The peptide is often used in conjunction with a healthy diet and exercise regimen to enhance fat loss. It's important to note that AOD-9604 is not FDA-approved for weight loss, and its use should be under medical supervision.

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While AOD-9604 presents an intriguing option for targeted fat loss with a favorable safety profile, its clinical efficacy remains inconclusive. Individuals considering its use should consult healthcare professionals to weigh potential benefits and risks, especially given the lack of FDA approval for weight loss applications.

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AOD-9604 dosing can vary depending on the form and the goal, but here’s a detailed breakdown based on what is commonly used in clinical and research contexts:

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Typical Dosing for Fat Lose:

​•Subcutaneous Injection: Most protocols suggest 250–500 mcg per day, usually administered once daily. Some advanced protocols split the dose into two injections of 250 mcg, taken in the morning and evening to potentially maximize fat metabolism.

• Oral/Lozenge Form: Dosage ranges from 300–500 mcg daily, though absorption is generally lower than injections. Consistency is key with oral forms.

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Cycle Duration:

• Commonly used in 8–12 week cycles, followed by a 2–4 week break before restarting. This allows the body to respond without building tolerance or causing receptor downregulation.

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Administration Tips:

1. Timing: Many users prefer taking AOD-9604 before meals to potentially enhance fat metabolism. Others take it before workouts.

2. Injection Sites: Subcutaneous injections are usually done in abdominal fat, thighs, or upper arm. Rotate sites to avoid irritation.

3. Storage: Keep reconstituted peptide in the fridge and protect from light. Use within 2–4 weeks depending on manufacturer guidance.

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Important Notes:

• AOD-9604 is not FDA-approved for weight loss, so all dosing recommendations are based on research studies, clinical trials, and anecdotal reports from peptide therapy practitioners.

• It is generally considered safe, but consult a healthcare professional before use, especially if you have metabolic disorders, diabetes, or other health conditions.

• Combining with diet and exercise significantly improves outcomes; AOD-9604 alone is unlikely to produce dramatic fat loss.

 

AOD-9604 Injectable Protocol:

•Goal: Fat loss / metabolism support
Form: Subcutaneous injection

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•Time of day dose- Morning (fasted) use 50 mcg. Inject subcutaneously in the abdominal area or thigh. Fasting may enhance fat metabolism.
 

•Evening does (optional) Use 250 mcg. Only if splitting dose can be before dinner or pre-workout in the evening.

Cycle:

• 8–12 weeks continuous use.

• 2–4 week break between cycles.

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Administration Tips:

• Rotate injection sites to prevent irritation.

• Reconstituted peptide should be stored in the fridge, away from light, and used within 2–4 weeks.

• Inject at a 45-degree angle into subcutaneous fat.

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AOD-9604 Oral/Lozenge Protocol:

Goal: Fat loss / metabolism support
Form: Oral lozenge or sublingual

Time of Day dose -Morning

300–500 mcg.

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Take before breakfast; hold under the tongue until fully dissolved for better absorption.

​Pre-workout (optional) 250–300 mcg. May enhance fat oxidation during exercise.

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Cycle:

• 8–12 weeks continuous use

• 2–4 week break before next cycle

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Administration Tips:

• Consistency is important; take at roughly the same times daily.

• Combine with regular exercise and a balanced diet to maximize fat loss.

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Additional Tips:

1. Hydration: Keep hydrated to support metabolism.

2. Diet: A moderate calorie deficit plus protein intake can improve results.

3. Monitoring: Track body composition rather than just weight for better results.

4. Stacking: Some protocols combine AOD-9604 with other peptides like CJC-1295 without DAC for enhanced growth hormone pulse support, but do this only under medical supervision.